Quantitative Vitamin D Assay Kit

Vit D Gold Medi Test Plus 250 ELISA kit is used for the quantitative measurement of Vitamin D in human serum and plasma samples. This kit is only for IVD (In Vitro Diagnostic) use and is intended for use in medical diagnostic laboratories.

Applications of the Quantitative Vitamin D Assay Kit

This kit is used for:

• Diagnosing Vitamin D deficiency.
• Assessing the body’s calcium and phosphorus status.
• Monitoring treatments for bone metabolism disorders like osteoporosis and rickets.
• Investigating potential Vitamin D toxicity.
• It also applies to evaluating diseases related to immune disorders and calcium metabolism.

How to Use the Quantitative Vitamin D Assay Kit

1. Prepare Wells: Designate wells for controls and standards. Place any unused wells with desiccant in the provided special bag.
2. Add Samples: Dispense 25 microliters of samples, controls, and standards into the wells using separate pipettes.
3. Add Conjugate: Add 50 microliters of diluted conjugate solution to all wells and gently shake the plate.
4. Incubate (1st): Cover the plate and incubate for 60 minutes at 37°C.
5. Add Biotin: After incubation, without washing, add 50 microliters of biotin and shake for 60 seconds.
6. Incubate (2nd): Cover the plate and incubate for 30 minutes at 37°C.
7. Wash: Wash the plate 5 times with wash solution (300-350 microliters) and then dry it.
8. Add Substrate: Add 100 microliters of substrate solution to the wells.
9. Incubate (3rd): Incubate the plate for 15 minutes at room temperature, away from light.
10. Add Stop Solution: Add 50 microliters of stop solution to the wells and mix gently; the blue color will change to yellow.
11. Read Plate: Read the plate at 450 nm with a 630 nm reference filter (within a maximum of 10 minutes after stopping the reaction).

Normal Values

The normal ranges for Vitamin D levels in the serum of healthy individuals obtained by repeated ELISA tests are as follows. However, it is suggested that each laboratory establish its own reference range.

Sensitivity: The detectable Vitamin D in this kit is 4 ng/mL.