Hepatitis B virus (HBV) is an enveloped, double-stranded DNA virus belonging to the Hepadnaviridae family. Along with Hepatitis C virus (HCV), it is a major cause of blood-borne hepatitis. HBV infection can range from mild, asymptomatic forms to fulminant hepatitis and severe chronic liver disease, sometimes leading to cirrhosis or liver cancer.
To diagnose and classify hepatitis B infection, it’s essential to identify various serological markers across three stages of infection: incubation, acute phase, and recovery. The most crucial marker is Hepatitis B surface antigen (HBsAg), previously known as Australia antigen. HBsAg possesses a common “a” determinant across all viral subtypes and is immunologically divided into two subgroups (ay and ad). HBV has 10 main serotypes and 4 known HBsAg subtypes (ayw, ady, adw, ayr). HBsAg is detectable 2 to 4 weeks before abnormal ALT levels and 3 to 5 weeks before the onset of clinical symptoms.
Serological detection of HBsAg, particularly using the ELISA method with a hepatitis virus detection kit, is a key tool for clinical screening, timely diagnosis, and preventing HBV transmission.
The Aria Mabna Tashkhis HBSAg ELISA kit is designed for the qualitative detection of Hepatitis B virus surface antigen (HBSAg). This kit uses an ELISA system to detect HBV infections and can identify acute, chronic, and early stages of the disease. This tool is widely used for screening, prevention, and blood safety testing.
The HBSAg ELISA kit is used for detecting Hepatitis B virus surface antigen in human serum or plasma. This kit is applicable for diagnosing and controlling HBV infections in diagnostic laboratories, screening general populations, and blood banks, and helps in the rapid identification of early stages of the disease.
Patient serum or plasma samples are placed in the plate wells.
During incubation, antigens present in the sample react with HRP reagents and chromogenic solutions.
The wells are cleaned with a wash solution, and chromogenic solutions are added.
The color produced by the enzymatic reaction, proportional to the amount of antigen in the sample, is evaluated.
Results are recorded and interpreted at a wavelength of 450 nm.
Use only by trained professionals.
Do not use expired reagents or samples.
Severely lipemic, hemolytic, or contaminated samples may yield inaccurate results.
Kit solutions must reach room temperature before use.
Laboratory and washing equipment should be calibrated periodically.
Protect plates from scratches and hand contact to ensure more accurate results.
Wells should not dry out during the washing steps.
Avoid testing samples that have been repeatedly frozen and thawed.
Carefully read the kit instructions and do not alter the test procedures.
