The quantitative B-hCG hormone measurement kit in serum is a vital tool for accurately measuring human chorionic gonadotropin (hCG). This glycoprotein hormone has two chains, alpha and beta, with the beta chain determining its specific characteristics.
After fertilization, hCG is secreted by trophoblast cells. It maintains the corpus luteum, boosting progesterone and estrogen production, which creates the necessary conditions for implantation and the continuation of pregnancy. The most important clinical application of this hormone is confirming pregnancy.
Additionally, hCG measurement is effective in determining gestational age, diagnosing ectopic pregnancy, assessing the likelihood of miscarriage, and identifying preeclampsia. In abnormal pregnancies, the hormone’s concentration is lower than normal. Conversely, elevated hCG is observed in certain tumors, such as hydatidiform mole, choriocarcinoma, and tumors of the ovary, testicle, breast, lung, etc., making its measurement highly important in the diagnosis and monitoring of treatment for these conditions.
The Gold Medi Test Plus Quantitative B-hCG Hormone Measurement Kit in serum is designed using the ELISA method and allows for accurate measurement of human chorionic gonadotropin (hCG) hormone in serum or plasma samples. This kit is intended for In Vitro diagnostic use to assess pregnancy status and B-hCG-related conditions. By utilizing an immunoenzymatic sandwich method and monoclonal antibodies, it ensures the accuracy of laboratory results.
This ELISA kit is used for confirming pregnancy, approximate gestational age determination, identifying ectopic pregnancy, assessing the likelihood of miscarriage, and monitoring trophoblastic tumors and hydatidiform mole. It is also used in evaluating response to treatment for conditions associated with abnormally increased or decreased hCG levels.
Allocate one well for the control, five wells for the standards, and wells for the number of samples. Place the remaining wells with desiccant inside the special bag and seal it. It’s best to test standards in duplicate.
Add 50 microliters of sample, standard, and control to the wells. Use a separate pipette tip for each. Add 50 microliters of assay buffer to all wells and gently shake the plate for 10 seconds. Cover the plate with a seal and incubate for 5 minutes at room temperature (20–25°C). Wash the wells 5 times with 300–350 microliters of wash solution (20–40 seconds each time). Then tap the plate on a paper towel to remove excess moisture. Add HRP conjugate: Add 100 microliters of conjugate to all wells and gently shake for 30 seconds. Re-incubate the plate for 5 minutes at room temperature. Repeat the washing step as in step 5. Add chromogen solution (TMB): Add 100 microliters of TMB solution to the wells. Incubate the plate for 5 minutes at room temperature, away from light, for the enzymatic reaction to produce a blue color. Add 50 microliters of stop solution to each well and mix gently. The blue color will change to yellow. Read the plate at a wavelength of 450 nm with a 630 nm reference. Do this within a maximum of 10 minutes after stopping the reaction.
In healthy non-pregnant individuals, serum hCG concentration is usually less than 5 mIU/mL. In a normal intrauterine pregnancy, hCG concentration is measurable approximately 1.5-1 week after conception and is about 1 mIU/mL. The concentration of this hormone doubles every 1.5-3 days during the first 6 weeks of pregnancy. In late first trimester, hCG concentration reaches its peak, approximately 100,000 mIU/mL. Then, from the early second trimester, its level slowly decreases. It’s worth noting that the concentration of this hormone is approximately double in twin or multiple pregnancies. In ectopic pregnancies, the concentration of this hormone is lower compared to normal intrauterine pregnancy. Samples with B-hCG concentrations in the range of 5-10 mIU/mL are considered suspicious for pregnancy, and repeat testing after 48 hours is recommended. Samples with hCG concentrations exceeding 25 mIU/mL are considered positive. In normal intrauterine pregnancies, the reported B-hCG levels in reference texts are as follows:
It’s important to note that the above table is a general guide, and it is recommended that each laboratory determines its own normal reference values by measuring B-hCG concentrations in healthy and pregnant individuals and uses these for interpreting results. The measurable range of B-hCG concentration with this kit is 1-1,000,000 mIU/mL.
