Use separate pipette tips for each negative control and positive control sample. Incubate the plate for 30 minutes at 37°C.
Discard the solutions from the wells and wash the plate 5 times with wash buffer, using a wash bottle or an automated plate washer. For each wash, fill the wells completely with buffer, let it stand for 30 seconds at room temperature, then completely empty and thoroughly dry by tapping on absorbent paper. Avoid insufficient washing and leaving residual solution in the wells.
Within 30 minutes after adding the stop solution, read the optical absorbance of the wells at 450 nm using an ELISA reader. If the instrument has a reference filter, use 620-630 nm as the reference wavelength.
Note: If a blank well (color control) was used in the assay, its optical absorbance should be subtracted from all other results.
Tip: If a sample’s result is greater than the cut-off, the sample is considered positive. (Please interpret the response.)
The Human Immunodeficiency Virus (HIV) Detection Kit is a two-step ELISA (Enzyme-Linked Immunosorbent Assay) tool designed for the qualitative detection of anti-HIV-1 and anti-HIV-2 antibodies in human serum or plasma samples. This assay is used as an aid in the clinical diagnosis of HIV infection and the etiological agents of Acquired Immunodeficiency Syndrome (AIDS).
This ELISA kit is used for the detection of HIV-1 and HIV-2 antibodies in the serum or plasma of individuals suspected of HIV infection. It helps identify serological evidence of infection and provides results as a supplementary tool for the clinical diagnosis of HIV-infected patients. This assay is also used for blood screening and preventing disease transmission in blood banks.
The patient’s serum or plasma sample is placed into the microplate wells. During the first incubation, specific HIV antibodies, if present, are captured in the wells. The wells are washed, and recombinant antigens conjugated to HRP enzyme are added. During the second incubation, a colorimetric reaction occurs, where the intensity of the color is proportional to the amount of antibody in the sample. Results are evaluated using a plate reader at a wavelength of 450 nm.
